BIOSTRAINZ™ has been specifically designed for wastewater treatment applications and has excellent wastewater treatment and odor control properties. BIOSTRAINZ™ deals with a wide range of organic waste streams and is particularly well suited to nitrification and de-nitrification applications

Key Value Benefits from using BIOSTRAINZ

1-Reduced operating expenses

1.1  Reduction in sludge haulage and disposal

One of the key benefits of the introduction of BIOSTRAINZ™ will be the significant reduction in sludge biomass. The reduction can be up to 85%, which will result in significant savings in sludge haulage and disposal fees. Secondary cost savings can occur in reduced labor and capital costs required for residual sludge handling.

1.2 Energy cost savings

BIOSTRAINZ™ has the ability to lower required effluent detention times and in some applications the need for mechanical agitation. This can result in lower energy costs in treating the influent. Quantifiable energy savings from lower demands on equipment per unit of influent and/or being able to switch off equipment altogether can be realized.

1.3 Reduction in chemical additive costs

Chemical additives, such as flocculants, can be reduced due to the lesser biomass contained in the sludge resulting in direct savings in chemical costs. BIOSTRAINZ™ also has antifungal properties lessening the dosing volumes of potential disinfectants resulting in additional savings. The reduction of chemical use will also create an additional benefit to operators as they will handle less of these chemicals.

1.4 Increased longevity of treatment plant equipment

Addition of BIOSTRAINZ™ can reduce strain on equipment from handling higher sludge volumes. BIOSTRAINZ™ will also reduce hydrogen sulfide by up to 17% increasing longevity of plant equipment. This should translate to lower maintenance costs of treatment plant equipment.

2. Reduction in odor

BIOSTRAINZ™ has the added benefit of reducing odors emitted from wastewater treatment applications. This is a significant benefit when residential housing encroaches upon treatment facilities.  Improvement in odor emission can have a positive impact resulting in less complaint from the community and less public pressure on wastewater treatment plant owners and professionals.

3. Increased capacity/capital avoidance

I The addition of BIOSTRAINZ™ will result in increased plant capacity due to lower biomass in the process, as well as decreased contact time. This will enable higher volumes of wastewater to be treated with the same equipment, resulting in capital avoidance.

4. Increased process stability

BIOSTRAINZ™ may assist in increasing plant stability as there is less reliance on maintaining a biomass. The result is less time must to be spent on monitoring and tweaking plants to retain stability.

5. Potential to reduce classification of sludge waste

In some instances the efficiency of BIOSTRAINZ™ technology will remove a significant amount of pathogens and volatile organic content from sludge waste. This may result in the reclassification of waste products to less hazardous criteria. This may make sludge waste suitable for salable commercial applications rather than waste disposal.

6.  Suitable for all Biological treatment plants

BIOSTRAINZ™ is a natural product that lends itself to biological treatment processes and hence there are no special requirements for the addition of BIOSTRAINZ™ to a biological treatment plant. 

7. No bolt on technology/capital costs required for implementation

BIOSTRAINZ™ is simple to dose on an ongoing process and can be done manually or by a relatively simple batching process. The result is there are no capital implementation costs associated with implementation.

8.Reduction in strength of effluent

The efficiency of BIOSTRAINZ™ will significantly reduce BOD, COD, SS, TKN, and turbidity loadings in effluents.  This will help effluent to meet waste treatment licensing requirements.

Key Mechanism of Actions

Dosing of BIOSTRAINZ should take place as high upstream in the influent flow as possible. This allows for maximum contact time for the high speed enzymes to break down pollutant and nutrient molecules. It also allows maximum strength culture to be present in the aeration chamber. Simple two tank pre-activating dosing systems are the most effective in introducing the technology into the influent. This represents a very minimal capital requirements and deliver the most economical results to plant operation. For very low flow plants the technology is applied by simply hanging the BIOSTRAINZ in filter bag in influent.
In sewage operation, the optimal dosing point may be within collection network. Dosing at key rising mains or pumping stations throughout the collection system will result in substantially treated influent arriving at the treatment plant.

When BIOSTRAINZ is used in a plant there is less reliance on processes such as BNR or anaerobic or anoxic chambers. These stages are introduced to assist with nutrient uptake by bacterial biomass. As the BIOSTRAINZ treatment technology is not dependent on uptake by bacterial biomass, the role for these stages is reduced. Their presence in stream are not however detrimental to BIOSTRAINZ as it operates effectively as a facultative anaerobic and will continue the action through anaerobic and anoxic stages

Nitrogen is typically removed from waste water through a combination of uptake into bacterial cellular material, microbial digestion and sedimentation. Removed from the plant as a component of sludge the majority of the nitrogen then completes its transformation through the nitrogen cycle external of the plant. BIOSTRAINZ™ has the ability to enzymatically accelerate the completion of the nitrogen cycle to remove a large portion of nitrogen in the form of nitrogen gas (N 2) within the confines of the plant. This process requires amonification, Nitrification, Dentrification to ultimately break the nitrogenous compounds in influent into CO 2 (Carbon Dioxide), H2O (Water) and N2 (Nitrogen Gas(. This action is evidenced by higher than normal in-process measures of ammonia, nitrites and nitrates demonstrating progressive break down of the nitrogenous waste at rates much faster than are otherwise possible. Despite these higher in-process measures, final effluent is typically lower in total nitrogen, ammonia and nitrate than traditional plant operations provides, due to the enhanced biological efficiency in the aeration chamber.

Unlike nitrogen compounds which can be broken down and off-gassed as nitrogen gas (N2-See Nitrogen management), Phosphorus can not be converted into gaseous state for removal at temperatures in waste water environment. As a result, Phosphorus is most effectively treated in plants adopting BIOSTRAINZ technology through co-aggulationand Sedementation. The Phosphorus then is removed from the plant as phosphate. In large scale plants, this is commonly achieved through Alum Dosing. Whilst there has been a trend to BNR (Biological Nutrient Removal) in recent years targeting uptake of phosphorus by phosphorus favoring bacteria strain. The benefits of this are far outweighed by overall plant efficiencies that enzymatic treatment with BIOSTRAINZ offers

Sequencing Batch Reactors (SBR)/Biological reactors
Similar to RAS systems, a portion of activated sludge is retained in SBR tank. When BIOSTRAINZ is introduced to the plant it is recommended that 90-95% of tank volume is decanted in each batch. This retain a small quantity of low strength enzymatic inoculants sludge.

Due to the lower biomass in the process, BIOSTRAINZ will typically provide improved settings characteristics Lower Sludge Volume Index (SVI).

This is a significant benefit when residential housing encroaches on treatment facilities. This improvement in odor emission can have a positive impact resulting in less complains from the community

A very common technology process for waste water treatment is the use of return activated sludge. This process of re-circulating a highly cultured, mature microbial biomass has been the mainstay of nutrient removal in waste water treatment for several decades. This process not only reduces waste it also consumes substantial energy for aeration and creates the high bio-solids output of traditional plants.
The BIOSTRAINZ™ high speed enzyme technology changes the role of the RAS technology in the plant process and operation. RAS is no longer required to re-circulate a high volume of biomass when BIOSTRAINZ ™ is added into a plant as the plant no longer relies on the biomass to remove nutrients as this is done enzymatically. In-process measures of quality, through an activated sludge plant, typically shows a worsening of quality prior to improvement, due to the reintroduction of the concentrated activated sludge. This inherent inefficiency can be avoided if the raw influent can be treated without the addition of activated sludge however previous technologies have failed to achieve adequate nutrient removal.
When using BIOSTRAINZ™ – best results are achieved by allowing the high speed enzymes to operate as effectively as possible by not re-introducing nutrient, biomass or contaminants back into the waste stream. In process terms, this means wasting all accumulated sludge and biomass from the system shortly after the introduction of BIOSTRAINZ™. Thereafter the plant operates enzymatically. At this time the RAS system is employed to return approximately 5% of inflow volume to assist with enzymatic inoculation.

Sludge is removed from the operation as filter cake (industrial) or bio-solids (sewage). In certain industrial treatment plants, the biological activity is often low due to the type of contaminant being treated, and as a result of low resonance times. Filter cake is a result of high coagulation and flocculation additives utilized to remove suspended solids and contaminants. The BIOSTRAINZ™ enzymes rapidly remove the contaminants at the molecular level including many suspended solids, hence the need for flocculation and coagulation is substantially reduced, resulting in reduced filter cake volume. In sewage treatment and some industrial processes, the majority of bio-solids produced are dead bacterial cells that were formed to remove the nutrients (carbon, nitrogen, phosphorus etc) from the sewage through microbial digestion for energy and in the creation of new bacterial cellular material. As a high speed enzyme technology,

Waste water treatment typically requires high levels of aeration to sustain suffient oxygen levels to support high levels of biomass and oxidization of waste material. This high oxygen demand leads directly to high electricity consumption for aeration.
By removing the reliance on biomass to remove nutrients and contamination, oxygen demand is substantially and rapidly reduced. The key contributors to the reduced oxygen demand are reduced microbial respiration due to lower levels of biomass, rapid removal of organic waste hence lower oxidization demand, lower microbial cell formation. The result of this is a natural increase in dissolved oxygen (DO) levels. Where employed, auto DO monitoring equipment will manage aeration allowing for BIOSTRAINZ™ technology to deliver energy savings often in excess of 50%. Where such technology is not employed, manual monitoring and adjustment of aerator run time and speed are required to realize energy savings. This not only provides direct cost savings to the plant but may offer options to generate carbon credits or obtain other forms of incentive for the adoption of energy saving technologies.

Key Principles of Use


There is a wide range of effective concentrations or ‘doses’ of BIOSTRAINZ for certain applications, however the optimum range is generally between 2 and 10 milligrams per liter of volume—that is milligrams of BIOSTRAINZ per liter of wastewater or substrate. This is roughly equivalent to parts per million.

Contact Time

To maximize the BIOSTRAINZ treatment program, BIOSTRAINZ should be kept in contact with the target substrate to the greatest extent possible. This means that when applying BIOSTRAINZ to a large volume of wastewater, such as a large lagoon, tank or vessel, it is important to maximize the distribution of BIOSTRAINZ throughout that medium. Additionally, the frequency of BIOSTRAINZ dosing should match or exceed residence time within the wastewater system. For example, if a septic tank has a residence time of 14 days, then BIOSTRAINZ should be applied at least every 14 days. If a grease trap fills and empties completely every day, then daily dosing or continuous with BIOSTRAINZ is advised.


BIOSTRAINZ can operate effectively in a wide range of temperatures. However, the catabolic break-down of organic waste molecules into inert compounds slows significantly as the temperature of the substrate approaches the freezing point (32°F/0°C). There is a similar loss of effectiveness at temperatures above approximately 140°F (60°C). The ideal operating temperature is between 50°F and 120°F (10°C to 49°C). This broad operating range makes BIOSTRAINZ a suitable treatment for effluent lagoons, sludge drying beds and effluent storage basins in most climates during most times of the year.


BIOSTRAINZ operates within a broad pH range, from 2.5 and 9.5, but the optimum pH is between 3.5 and 8.5 with neutral preferred.

Oxygen Levels

When using BIOSTRAINZ in an aerobic process the performance of BIOSTRAINZ can be improved by higher levels of dissolved oxygen. Optimum levels in aerobic processes are DO>1.5mg/L, however BIOSTRAINZ is equally effective in anaerobic processes with lower DO.

Pre-Activation of BIOSTRAINZ™

BIOSTRAINZ-FOG is packaged in a freeze-dried powder form and delivered in vacuum-sealed bags. The powder must be pre-activated by mixing into solution with water before being applied to the wastewater or target medium.

This step allows BIOSTRAINZ to properly culture and forms a high concentration of catalysts. Several factors govern the effectiveness of this BIOSTRAINZ dose pre-activation phase.

Pre-Activation Period

The appropriate time period for preparing a BIOSTRAINZ culture is between 12 and 24 hours. Less time may will result in a less optimal concentration of catalysts, while too much time may cause BIOSTRAINZ to run out of its nutrient source and lose viability. Each package contains a proper amount of nutrient to sustain BIOSTRAINZ during the pre-activation phase.


For pre-activation, BIOSTRAINZ should be mixed into solution with water at a concentration between 2,000 and 10,000 milligrams of BIOSTRAINZ per liter of water (parts per million). The ideal concentration is approximately 5,000 mg/L.

Because BIOSTRAINZ is packaged with its own nutrient source, it is important to use a relatively nutrient-free source of water for pre-activation mixing.


The range of optimum temperature for pre-activating BIOSTRAINZ is not as broad as its effective temperature range once activated. It is ideal to keep the pre-activation culture at a temperature above 60°F (16°C ) and below 100°F (38°C ).


The BIOSTRAINZ pre-activation culture requires adequate oxygen. In some cases—particularly when large volumes of product are being mixed into a pre-activation solution—some mechanical means of aeration is advisable. In most cases it is sufficient to simply leave the solution container uncovered and exposed to ambient air throughout the pre-activation period.

Quality of Water Used

The BIOSTRAINZ activation is a biological process. It is important that the water used does not contain any harsh chemicals, such as microbial inhibitors, that would prevent the growth of the BIOSTRAINZ Culture.


Please refer to the Wastewater Treatment Application Manual for BIOSTRAINZ-FOG dosing programs.