BIOSTRAINZ™ wastewater application products are packaged in a freeze-dried powder form, delivered in vacuum-sealed bags. The powder must be pre-activated by mixing into solution with water before being applied to the wastewater or target medium. This step allows BIOSTRAINZ to properly culture and form a high concentration of catalysts. Several factors govern the effectiveness of this BIOSTRAINZ dose pre-activation phase:

Pre-Activation Time. The appropriate time period for preparing a BIOSTRAINZ culture is between 12 and 24 hours. Too little time will result in a less optimal concentration of catalysts, while too much time may cause BIOSTRAINZ to run out of its nutrient source and lose viability. Each package contains a proper amount of nutrient to sustain BIOSTRAINZ during the pre-activation phase.

Concentration. For pre-activation, BIOSTRAINZ should be mixed into solution with water at a concentration between 2,000 and 10,000 milligrams of BIOSTRAINZ per liter of water (parts per million). The ideal concentration is approximately 5,000 mg/L. Because BIOSTRAINZ is packaged with its own nutrient source, it is important to use a relatively nutrient-free source of water for pre-activation mixing.

Temperature. The range of optimum temperature for pre-activating BIOSTRAINZ is not as broad as its effective temperature range once activated. It is ideal to keep the pre-activation culture at a temperature above 60°F (16°C ) and below 100°F (38°C ).

Oxygen. The BIOSTRAINZ pre-activation culture requires adequate oxygen. In some cases—particularly when large volumes of product are being mixed into a pre-activation solution—some mechanical means of aeration is advisable. In most cases it is sufficient to simply leave the solution container uncovered and exposed to ambient air throughout the pre-activation period.

Quality of water used. BIOSTRAINZ activation is a biological process. It is important that the water used does not contain any process that would inhibit the growth of the BIOSTRAINZ culture. Water with antimicrobial agents should not be used. 

Pre-Activation Steps

Equipment:
• 1 x 100 Liters Clean Tank
• 2 x Fish Tank Air Pumps (recommended that each is to be equipped with 2-Air Outlets).
• 1 x Fish Tank Cleaning Motors (Filters are to be completely removed).
• 1 x Temperature Gauge.
• 1 x pH Gauge.

Procedures
• Install the fish tank cleaning motor at the bottom of the tank (make sure the filters are completely removed).
• Install the outlets of one of the fish tank air pumps at the bottom of the Water Tank.
• Fill the water tank with 95 liters of standard tap water.
• Keep the fish tank cleaning motors and the fish tank air pumps running for 120-minutes before applying the dose
• After two hours of continuous aeration to the water, a dose of 1,000 grams is to be applied for every 95 liters of water.

1. Once the dose has been applied, the second fish tank air pump is to be applied to the tank.

2. After starting the 4th hour of the application of the product into the water, the pH value and the temperature are monitored every hour.

3. Once the temperature starts rising above 30° Celsius; this is an indication that the solution is starting to reach the maturity time and thus both the pH and the temperature are to be monitored every 30-minutes (not every hour), and the later applied air pump is to be stopped. Only the pump at the bottom is to be kept running with the cleaning motor.

4. Once the pH value starts moving below 7 and/or the solution temperature starts reaching towards the 37° Celsius this means that the solution is in its prime condition and is to be applied to the media subject of treatment within a maximum of one hour, and in this case the air pump is to be stopped once any of the above conditions (pH = 6 or temperature = 37 degrees) one of the two outlets of the air pump is to be removed.

5. If the solution reaches this condition and is kept unused for more than two hours, the pH value will rise again, which is an indication that some of the bacterial strains have started dying and the solution will lose a good portion of its efficiency.

Special Cases

1. The above referred conditions would usually take anywhere between 12-18 hours for completion of pre-activation depending on the quality of the pre-activating water, room temperature and strength of the air pumps being used for aeration.

2. If in a special case the pre-activation is required to be completed within a very short duration, 50mL of Glucose should be added to the solution with the water filling at the beginning stage, which will reduce the pre-activation time to no more than 3-5 hours. However, this application makes the pre-activation procedure very aggressive and if the solution is not used quickly upon reaching the prime point, multiple strains of the bacteria will be lost in the solution.